I’ve noticed myself that there’s usually around 0.5ml missing from vials and I get emails about that from others as well.
Since its Friday and I’m not in the mood to be productive I decided to use the equipment from my lab and do some measurements. Before anyone gets excited, I don’t work in a chemical lab, but in an electronics lab. I do systems engineering and programming.
I only had 5ml Syringes and 21G needles (1-inch-long) available, so used that in this experiment.
The purpose of this experiment is to determine the volume of liquid that is wasted when we inject. I don’t know the density of the oils used to make injectables and I don’t have the equipment to test it either.
Luckily I know the density of liquid water is 1000kg per m3 at 4 °C and a m3 is exactly 1000L, so 1kg of water is 1L in volume at 4 °C.
So I’ll be using the weight of water to determine the volume.
The scale I used is accurate to the nearest milligram. (Resolution of 0.001g). The scale's cover was closed for measurements to ensure accuracy, but the cover was opened for the photos below.
1: The first step was to calibrate the scale.
2: Then I measured the needle and syringe together after removing them from their packaging. I also removed the cap from the needle. The combined weight was: 4.169g
3: I then used the needle and syringe to draw sterile water (not saline!!) that I cooled to 4 °C.
4: I tapped out all the air bubbles and squirted all the water out of the syringe. I pressed hard onto the plunger of the syringe to make sure that there was nothing left.
5: I measured the needle and syringe again and this time the weight was 4.265g, so 0.096g heavier.
So in total I lost 0.096g of water that I couldn’t get out of the needle and the syringe’s spout.
0.096g of water at 4 °C will give us a volume of 0.096ml.
Let’s say you use your vial of 10ml to do 5 injections you will waste a total of 0.48ml.
This is a very simple experiment to do, so feel free to test it yourself if you don’t believe me. I tried it twice and the difference the second time was 0.09ml as well.